Recombinant Human EPOα

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產(chǎn)品型號：abs00840
廠商性質(zhì)：生產(chǎn)廠家
更新時間：2024-07-07
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重組蛋白原代細(xì)胞分離細(xì)胞培養(yǎng) 細(xì)胞檢測細(xì)胞/組織處理外泌體研究其它動物血清 3D/類器官培養(yǎng)

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品牌	absin	貨號	abs00840
規(guī)格	500IU	供貨周期	一周

概述
形態(tài)	Lyophilized from a 0.2 μm filtered concentrated solution in sodium citrate buffer (1 liter of ddH2O containing 5.9 g of sodium citrate, 5.8 g of sodium chloride and 0.06 g of citric acid).
來源	CHO
氨基酸序列	APPRLICDSR VLERYLLEAK EAENITTGCA EHCSLNENIT VPDTKVNFYA WKRMEVGA VEVWQGLALL SEAVLRGQAL LVNSSQPWEP LQLHVDKAVS GLRSLTTLLR ALGAQKEAIS PPDAASAAPL RTITADTFRK LFRVYSNFLR GKLKLYTGEA CRTGDR
內(nèi)毒素水平	Less than 0.01 EU/μg of Recombinant HumanEPO-α as determined by LAL method.
活性	Fully biologically active when compared to standard. The Specific Activity was measured by the stimulation of reticulocyte production in normocyth-aemic mice and is no less than 1.5 × 10⁵ IU/mg.
Accession #	P01588
預(yù)期分子量	Mature human EPO, containing 166 amino acid residues, has a predicted molecular mass of approximay 21 kDa. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of 36-40 kDa in SDS-PAGE.

性能
溶解方法	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
保存方法	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
純度	> 98 % by SDS-PAGE and HPLC analyses.
Gene IDs	2056

參考文獻(xiàn)
1. Hanicki Z. 1988. Pol Arch Med Wewn, 80: 290-3.<br/>2. Haniu M, Narhi LO, Arakawa T, et al. 1993. Protein Sci, 2: 1441-51.<br/>3. Baranowska-Daca EandKsiazek A. 1994. Ann Univ Mariae Curie Sklodowska Med, 48 Suppl 3: 51-60.<br/>4. Chow KM, Szeto CC, Li PK. 2003. Am J Kidney Dis, 41: 266-7; author reply 7.<br/>5. Maurer MH, Schabitz WR, Schneider A. 2008. Curr Med Chem, 15: 1407-11.

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Recombinant Human EPOα

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