Rabbit Anti-IL-1 Beta Polyclonal Antibody

簡要描述：Rabbit Anti-IL-1 Beta Polyclonal Antibody WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （石蠟切片需做抗原修復(fù)）

產(chǎn)品型號：abs120224
廠商性質(zhì)：生產(chǎn)廠家
更新時間：2024-07-08
訪問量：989

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詳細(xì)介紹

品牌	absin	供貨周期	現(xiàn)貨
應(yīng)用領(lǐng)域	化工,生物產(chǎn)業(yè),能源

概述
別名	Rabbit Anti-IL-1 Beta Polyclonal AntibodyIL-1 β抗體;Catabolin; H1; IL-1β; Hematopoietin 1; IFN beta inducing factor; IL 1; IL 1 beta; IL 1B; IL1B; IL1F2; Interleukin 1 beta; Interleukin 1 beta precursor; LAF; OAF; Osteoclast activating factor; Preinterleukin beta; Pro interleukin 1 beta; IL1B_HUMAN; Interleukin-1 beta; IL-1 beta. IL-1B
宿主	Rabbit
特異性	IL-1 Beta
反應(yīng)種屬	Human;Mouse;Rat;
預(yù)測反應(yīng)種屬	Dog;Pig;Rabbit;Guinea Pig;
應(yīng)用	WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （石蠟切片需做抗原修復(fù)）
免疫原	KLH conjugated synthetic peptide derived from human IL-1beta:101-200/269
性能
濃度	1mg/ml
純化方法	affinity purified by Protein A
類型	Polyclonal Antibody
同種型	IgG
儲存/保存方法	Rabbit Anti-IL-1 Beta Polyclonal Antibody Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
存儲溶液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
研究領(lǐng)域	細(xì)胞生物免疫學(xué) 糖蛋白
靶標(biāo)
背景說明	The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008].
細(xì)胞定位	分泌型蛋白
UniProt	P01584
實驗結(jié)果圖
	Sample: Raw246.7 (Mouse)Cell Lysate at 40 ug Primary: Anti- IL-1 beta (abs120224) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17 kD Observed band size: 30 kD
	Tissue/cell: Human cervical carcinoma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min; Incubation: Anti-IL-1 Beta/IL-1B Polyclonal Antibody, Unconjugated secondary primary antibody 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
	Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min; Incubation: Anti-IL-1 Beta/IL-1B Polyclonal Antibody, Unconjugated secondary primary antibody 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
	Blank control (black line): A431(black) (The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with PBST for 30 min on room temperature) Primary antibody: Rabbit Anti-PTP1B antibody ;Dilution: 1μg /10^6 cells; Isotype Control antibody: Rabbit IgG . Secondary antibody: Goat anti-rabbit IgG-FITC;Dilution: 1μg /test.
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