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品牌 | absin | 貨號(hào) | abs04185 |
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規(guī)格 | 10ug | 供貨周期 | 一周 |
概述 | |
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描述 | Recombinant Human Brain-Derived Neurotrophic Factor is produced by our E.coli expression system and the target gene encoding His129-Arg247 is expressed. |
別名 | Brain-Derived Neurotrophic Factor, BDNF, Abrineurin |
形態(tài) | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 250mM NaCl, pH 7.2. |
來(lái)源 | E.coli |
氨基酸序列 | MHSDPARRGELSVCDSISEWVTAADKKTAVDMSGGTVTVLEKVPVSKGQLKQYFYETKCNPMGYT KEGCRGIDKRHWNSQCRTTQSYVRALTMDSKKRIGWRFIRIDTSCVCTLTIKRGR |
內(nèi)毒素水平 | Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test. |
Accession # | P23560 |
性能 | |
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背景 | Brain-Derived Neurotrophic Factor (BDNF) is a member of the neurotrophin family. Along with other structurally related neurotrophic factors NGF, NT-3 and NT-4, BDNF binds with high affinity to the TrkB kinase receptor. It also binds with the LNGFR (for low-affinity nerve growth factor receptor, also known as p75). BDNF promotes the survival, growth and differentiation of neurons. It serves as a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. BDNF expression is altered in neurodegenerative disorders such as Parkinson's and Alzheimer's disease. |
溶解方法 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
保存方法 | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
純度 | Greater than 95% as determined by reducing SDS-PAGE. |
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